The 2016 Regional Quiz Bowl Tournaments were hosted by six sites throughout North Carolina on March 5. 86 teams competed in 17 tournaments to be crowned Regional Champions and advance to the March 19 State Final hosted by NC School of Science and Mathematics of Durham. Shown below are the rankings of the advancing teams from each Regional:

West Regional
1 - Thomas Jefferson Classical HS
2 - Christ School HS
 
Charlotte Regional
1 - Metrolina Regional Scholars MS
2 - JM Robinson MS
3 - Thomas Jefferson Classical MS
 
1 - Providence HS
2 - Myers Park HS
3 - Ardrey Kell HS
 
Triad Regional
1 - Northwest Guilford MS
2 - Mount Mourne School
3 - Brown Summit MS
 
1 - Early College at Guilford
2 - High Point Central
3 - Atkins HS
4 - Jordan-Matthews HS
5 - Northwest Guilford MS
 
Sandhills Regional
1 - 71st Classical MS
2 - Pine Forest MS
 
1 - Terry Sanford HS
2 - Brunswick Early College
 
Triangle Regional
1 - Ligon MS
2 - Smith MS
 
1 - Raleigh Charter HS
2 - Carrboro HS
3 - Northwood HS
 
Northeast Regional
1 - NEAAAT
2 - Central Gates MS
 
1 - John A Holmes HS